Time-lapse imaging analysis demonstrated that LIN-32::GFP is restricted in the nuclei during interphase and that LIN-32::GFP evenly distributed in the cytoplasm of dividing Q.a and Q.p cells. The dynamic distribution of LIN-32 during asymmetric cell division is consistent with the role of LIN-32 as a transcription factor.
lin-32 starts its expression in Q neuroblasts and maintains the expression throughout Q neuroblast development. Interestingly,
lin-32 shows distinct expression patterns between Q.a and Q.p lineages. After Q neuroblast division, Q.a and Q.p appear to have the similar level of LIN-32::GFP fluorescence. However, in the end of Q neuroblast development, Q.a progenies have approximately five or four folds higher expression of LIN-32::GFP than that of Q.p progenies in the right side or left side of the animals.