nkat-1p::gfp was consistently expressed in head neurons with occasional weak transgene expression in the gonad and intestine. Based on their axonal projections and cell body positions, two of the
nkat-1p active neurons were identified as RMDV and RIM pairs of neurons, which are directly adjacent to
nmr-1-expressing AVA and AVD neurons, respectively. Only sporadic, weak mCherry expression could be observed in RIM. The third
nkat-1p active neuron situated dorsal to the nerve ring with an axon projection along the dorsal nerve cord was identified as RID. Thus, with the potential exception of RIM,
nkat-1 is likely expressed in neurons that lack NMDA receptors but are in close anatomical proximity to those that do (White et al., 1986).