Four constructs were made where GFP was fused to either initiation site or stop codon in order to determine if the splice variants are differentially expressed. Expression of the transformation vectors was driven under 1,951 bp of the KCNL-2 endogenous promoter. Confocal fluorescent imaging of transgenic lines expressing these constructs in the N2 background showed a neuronal expression pattern. expression of KCNL-2 when tagged at the latter stop codon (pkcnl-2kcnl-2
(taa2)::gfp) was detected in head neurons, the nerve ring (NR), motor neurons of the ventral nerve cord (VNC), the dorsal cord (DC) and tail ganglia. The pkcnl-2kcnl-2
(taa2)::gfp construct was expressed in many neuronal processes innervating the vulva while pkcnl-2gfp::(
atg2)
kcnl-2 shows expression in the VC4 and VC5 neurons of the egg-laying apparatus in addition to other neuronal processes innervating the vulva.