In wild-type animals, sax-7 is expressed in multiple tissues with robust SAX-7 accumulation in the nervous system. Expression was detected in pharynx, gonad, and the nerve ring and ventral nerve cord.
In wild-type animals, UNC-7 (representing all isoforms) is expressed throughout the nervous system, as well as in body muscles, particularly in the posterior.
All four anti-Lpin-1 antibodies detected a protein at the expected size in N2 embryonic extract that was reduced in lpin-1(RNAi) embryos to ~25% of the wild-type amount.