SPC-1/α is prominently expressed in both the spermatheca and SP-UT valvebeginning in L4 and extending into adulthood. In adults, SPC-1/α is also faintly expressed in the sheath.
Wild-type embryos were stained with AS1 and examined by immunofluorescence microscopy. In early embryos, alpha-spectrin is found at or near the cell membrane in most if not all cells starting at the earliest stage of embryogenesis, indicating that alpha-spectrin is maternally supplied. After morphogenesis and organogenesis initiates, alpha-spectrin localizes to the body wall muscle, hypodermis, pharynx, intestine, and developing nervous system. In epithelial tissues, alpha-spectrin is found at both the apical and basolateral regions of the membrane. This is unlike the localization pattern observed for beta-spectrin, which is found in only the basolateral region of the membrane. With the exception of the apical regions, the localization of alpha-spectrin overlaps with the distribution of beta-spectrin.
unc (uncoordinated)-29, which encodes a non-alpha receptor subunit, is expressed in AVA and RIB. Expressed also in sub-lateral neurons (possibly SMB/ Ds, SIBs or SIAs).
'hlh-2prox', an element from the hlh- 2 5' flanking region, is necessary and sufficient to promote transcription only in the four alpha and beta cells of the developing ventral uterus.
Relative RT-PCR using equivalent amounts of total RNA from different developmental stages. CE2FT-1 is expressed at all developmental stages with little relative difference in expression compared with alpha-actin.
Consistent with previous studies, the type-IV collagen α1 chain construct, EMB-9::mCherry, when expressed from the integrated transgene tkTi1[emb-9::mCherry], localized to the gonadal basement membrane in WT C. elegans.
Low level of transcription of the alpha-2 tubulin gene is observed in embryos gradually increasing in L1, L2 and finally reaching a maximum in L3. In L4 and adults transcription returns to basal embryonic levels.
The alpha-PKD-2 antibodies stained only male-specific sensory neurons in wild-type animals. Based on cell body positions, the PKD-2 protein is detected in the head CEMs, ray (except Ray 6), and hook HOB neurons of wild-type males.
LAM-3/Laminin alpha-A immunostaining was evident between the germ layers, including on most surfaces of the endoderm. In particular, LAM-3 localized around the surfaces of the endoderm row 8 cells, just before and as the somatic gonad precursors (SGPs) extended projections between the anterior surfaces of these endoderm cells and the posterior surfaces of the primordial germ cells (PGCs). A functional LAM-1-GFP fusion protein showed a pattern of localization similar to LAM-3, including around the gonadal primordium at later stages. Therefore, laminin basement membrane is present on endodermal surfaces where the SGPs migrate and extend projections around the PGCs.