Expr9343

egl-20 was mostly localized to the posterior half of L1 larvae, in a pattern that was already present at the comma stage of embryonic development. egl-20 was expressed in the rectal epithelial cells K, F, U and B, in the anal depressor muscle and in P11/12, which is in agreement with previous reporter studies (Whangbo and Kenyon, 1999). However, it was found that in L1 larvae, egl-20 was also expressed in the posterior ventral body wall muscle quadrants VL23 and VR24 and the rectal epithelial cell Y.

Expr9344

cwn-1 was mostly localized to the posterior half of L1 larvae, in a pattern that was already present at the comma stage of embryonic development. cwn-1 was mainly expressed in posterior body wall muscle cells and in the M cell descendants that give rise to body wall muscle cells and the vulva and uterine muscle cells. In addition, several cells were found to co-express cwn-1 including the anal depressor muscle, the body wall muscle quadrants VL23 and VR24 and P11/12. Interestingly, it was observed that the two lateral canal associated neurons (CANs) simultaneously induce cwn-1 expression during late L1, an expression that persists throughout larval development.