First detected in late embryonic stage and continues to be detected through to adulthood. Intensity is higher in animals undergoing ecdysis. Subcellular accumulation generally enriched in nuclei relative to cytoplasm. Cytoplasmic signal is enhanced in lateral seam cells during the molt periods. There is a period of cytokinesis where LIN-42::GFP is not observed. L3 molt: Higher intensity in seam cells, but most intense in V1.pppp. Cells that stain at this stage: H1.aa, V4.papp, H1.app, V5.pppp, H2.ppp V6.papp, V2.papp V6.pppp, V2.pppp, V3.papp, V3.pppp, V4.pppp. L4 molt higher intensity of staining observed in the cytoplasm of seam cell. LIN-42::GFP is present in lateral hypodermis,
hyp7 synctium which includes main body hypodermis and head and tail hypodermal cells.