Whole-mount immunofluorescent staining of wild-type animals with an antibody against UNC-7 revealed specific staining in both the nervous system and muscle. Along the processes of dorsal and ventral nerve cords, UNC-7 localizes to many small discrete puncta interspersed by infrequent larger puncta. Antibody staining against UNC-7 in a strain expressing a postsynaptic GABA receptor marker, UNC-49::GFP (oxIs22), further confirmed that UNC-7 puncta is present along nerve processes apposed to muscle. UNC-7 also localizes to discrete puncta outlining the surface of body-wall muscles, mostly at posterior muscles, and along muscle arms that extend perpendicular from toward the nerve cords.