Expr13342
PLST-1::GFP localized to both lamentous and punctate structures at the cortex of newly fer- tilized zygotes. PLST-1::GFP continued to be expressed ubiquitously during the first 5 h of embryogenesis, with prominent enrichment at cell-cell contacts, and during elongation, PLST-1::GFP was enriched in the buccal region of the epidermis, pharynx, and intestine. In adult hermaphrodites, PLST-1 expression was found in the socket cells of amphids, pharyngeal- intestinal valve, intestinal lumen, syncytial and cellularized gonad, vulval cells, intestinal-rectal valve, and socket cells of phasmids.The lamentous and punctate structures formed by PLST- 1::GFP in the newly fertilized zygote were also labeled by Lifeact::RFP, an F-actin reporter. The strong colocalization of the PLST-1::GFP and Lifeact::RFP signals, from before polarization, during polarity establishment and maintenance, and throughout cytokinesis, is evident in the representative line scans and from calculated Pearson's correlation coefficients with values >0.7 (n ≥ 8). This suggests that PLST-1::GFP associates with all cortical F-actin.