Consistent with previously reported bed-3
::gfp reporter expression, bed-3
mRNA (smFISH) was predominantly expressed in hypodermal cells and vulval cells. We next examined whether bed-3
is expressed earlier in the M lineage using ayIs6[hlh-8
::gfp]. We found that bed-3
is expressed in M daughters and descendants as well as SM cells. In L1 and L2 stages, the bed-3
smFISH signal appeared stronger in 2 M and 4 M stages compared to 8 M, 16 M and 18 M stages. In the L3 stage, bed-3
is expressed in SM cells near the vulval region of the animal.