Expr8172

Several T27A3.1pro::DsRed2-positive cells in the head region of the worms were also pan-neural::gfp-positive, demonstrating that T27A3.1 is indeed expressed in a subset of neurons. T27A3.1pro::DsRed2-positive neurons were first visible late in embryogenesis. Although there were some variations between worms within and between lines, up to seven neurons on each side of the head of the worm expressed T27A3.1. Authors were able to identify another pair of T27A3.1-expressing neurons along the body of the worm as CAN L and R.

Expr8173

To determine whether the T27A3.1pro::gfp neurons were chemosensory amphid and/or phasmid neurons, young adult worms were stained using the lipophilic dye, carbocyanine DiD. Colocalization of DiD staining with GFP was found in the head and the tail. Based on their positions, the chemosensory neurons expressing T27A3.1 are ASK L, ASK R, ASI L, and ASI R. In addition, at least two other T27A3.1-expressing neurons located anterior and posterior to the DiD-filled, ASH L neuron were identified as ADF L and ASE L chemosensory neurons. Other T27A3.1-expressing neurons, and their processes were found alongside DiD-stained chemosensory neurons and will require further analysis to be identified. In the tail region, T27A3.1pro::gfp was found in the more anterior DiD-filled neurons corresponding to PHB L and PHB R phasmid neurons.