To investigate whether the gfp-pest reporters were expressed in the dauer stage, the strains were induced to form dauers. In the
prml-4::gfp-pest strain, GFP-PEST was expressed dominantly in the seam cells of pre-dauers, but was not expressed at all in dauers. In the
prml-2::gfp-pest strain, GFP-PEST was also expressed in the seam cells of pre-dauers, although the expression level in the seam cells was variable from worm to worm and no GFP-PEST was expressed at all in dauers.