mir-84 temporal expression was observed in the hypodermal seam cells at the early L4 stage, similar to
let-7 seam cell expression.
mir-84 was expressed at the L1 stage in the Z1 and Z4 cells of the gonad, and during the L3 stage in the distal tip cells and uterine cells of the somatic gonad.
mir-84 expression was additionally observed in the sheath cells, the spermatheca, the uterine cells surrounding the eggs. In addition, the
lin-4 family member, mir- 237, and the
let-7 family member, mir- 84, were both expressed in the anchor cell and in the VPCs except P6.p at the mL3 stage. However, expression of
mir-84 in the vulval precursor cells appeared dynamic in nature and was detected in the P5.p, P6.p, and P7.p descendants but down-regulated in the P3.p, P4.p, and P8.p descendants by the end of the L3 stage (Johnson et al., 2005). It is interesting to note that expression of
mir-237,
mir-84, and
mir-48 was absent in the P6.p cell during the mid- to late L3 stage, and expression of
mir-237 and
mir-84 but not
mir-48 was present in the anchor cell.