SUR-7HGFP was expressed in most tissues with the notable exception of intestinal cells. Under a UV-light dissecting microscope, GFP was seen to be strongly expressed in the pharynx, some tail cells and the vulva. Under higher magnification, GFP was observed in many if not all neurons, muscles and hypodermal cells.
Asymmetric expression of imb-2 in AWCON was also observed in our initial assessment of the imb-2 expression pattern using imb-2 fosmid::GFP in which we fused GFP with the C terminus of IMB-2 by homologous recombination, that rescued imb-2(vy10) mutants.
NOS-2 protein is expressed sequentially during embryogenesis. First detected expression of NOS-2 in the 1-cell stage; at this stage, NOS-2 was present uniformly throughout the embryo. No NOS-2 expression was detected in 2- to 20-cell embryos. NOS-2 expression reappeared in the 28-cell stage in the cytoplasm of the germline blastomere P4. In some embryos, NOS-2 staining appeared to be concentrated in a few perinuclear foci. NOS-2 expression continued in P4 and its two daughters Z2 and Z3 until approximately the 200-cell stage. NOS-2 levels decreased sharply in later stages and were undetectable by the 550-cell stage.