Particularly intense
snf-5 EGFP signal was observed in intestinal cells INT1-9 of the alimentary canal. EGFP expression exhibited a more intensive and aggregated pattern in the epithelial cells of the mature gut, but more even and diffuse expression in the alimentary canal of younger worms. EGFP signal was detected in the pharynx in some, but not all, analyzed preparations. EGFP expression was also detected in the posterior cells of the alimentary canal, two cells of the rectal gland, and several cells that approximately colocalize with the positions of the DVA, DVB or DVC neurons. Further identification of these cells will require additional analysis. Expression of EGFP reporter was also identified in three pairs of amphid sensory neurons that are localized near the dorsa-anterior surface of the posterior pharyngeal bulb (pbp). Considering the localization to the lateral ganglia of head, the relative somatic positions identified via 3D reconstruction of confocal sections, the axonal projections, and the projections and shapes of dendrites visualized in a maximum projection of confocal stack these cells represent the ASI, ADF and ASK neurons. The ASI and ADF neurons exhibited intense labeling in the somata and along the axonal and dendritic projections. In contrast, ASK labeling was weak in the somatic regions and neuronal branches. EGFP expression was also detected in a population of small neurons at the dorsal surface of the anterior pharyngeal bulb.