unc-40p::unc-40::gfp genomic translational reporter gene is localized to all dividing ray cells before stage 1 of male development and only in ray structural cells from stage 3 onward.
smp- 2::gfp is strongly expressed in the male hook in close proximity to ray 1 in wild-type adult males. Expression was observed in rays 7, 8 and 9. No expression was observed in ray 1-6 lineages.
The alpha-PKD-2 antibodies stained only male-specific sensory neurons in wild-type animals. Based on cell body positions, the PKD-2 protein is detected in the head CEMs, ray (except Ray 6), and hook HOB neurons of wild-type males.