Expr86
Nuclear staining of about 60 cells at the end of dauer formation. Staining disappears several hours after the process is complete. All the seam cells stain and also hypodermal cells in the head and in the tail. The total number of cells shows some variability using promoters of different length. Positive identification of all cells is difficult because of poor fixation of dauers. Seam cells staining is consistent with location of CUT-1 in the cuticle in a ribbon underneath the alae and with immuno-EM pictures showing seam cells secreting CUT-1. Timing of expression consistent with northern, western, and immuno localization of CUT-1 in the cuticle.