The fluorescence from knock-in animals is fairly dim in the cell body and along the dendrite but is highly enriched in cilia. OSM-3-kinesin (OSM-3::GFP) was mostly distributed at the ciliary distal segments in knock-in animals.
Consistent with redundant functions of these motors in building the AWA cilium, KAP-1::GFP and OSM-3::GFP could be detected throughout the AWA cilia in wild-type animals. However, KAP-1::GFP was preferentially enriched in the short proximal AWA ciliary stalk with low expression levels in the distal ciliary branches, whereas OSM-3::GFP accumulated strongly at the distal tips of the AWA ciliary branches. A GFP-tagged OSM-6 IFT-B fusion protein was also present at low levels throughout AWA cilia but was enriched in the proximal stalk similar to KAP-1::GFP.
Consistent with redundant functions of these motors in building the AWA cilium, KAP-1::GFP and OSM-3::GFP could be detected throughout the AWA cilia in wild-type animals. However, KAP-1::GFP was preferentially enriched in the short proximal AWA ciliary stalk with low expression levels in the distal ciliary branches, whereas OSM-3::GFP accumulated strongly at the distal tips of the AWA ciliary branches. A GFP-tagged OSM-6 IFT-B fusion protein was also present at low levels throughout AWA cilia but was enriched in the proximal stalk similar to KAP-1::GFP.
Consistent with redundant functions of these motors in building the AWA cilium, KAP-1::GFP and OSM-3::GFP could be detected throughout the AWA cilia in wild-type animals. However, KAP-1::GFP was preferentially enriched in the short proximal AWA ciliary stalk with low expression levels in the distal ciliary branches, whereas OSM-3::GFP accumulated strongly at the distal tips of the AWA ciliary branches. A GFP-tagged OSM-6 IFT-B fusion protein was also present at low levels throughout AWA cilia but was enriched in the proximal stalk similar to KAP-1::GFP.