Consistent with prior reports (Huarcaya Najarro and Ackley, 2013; Steimel et al., 2010), we found that
fmi-1 was expressed throughout the C. elegans nervous system at all stages of development, revealed by a high-dose translational FMI-1::GFP reporter that contains the 2615 bp
fmi-1 promoter and the genomic
fmi-1 sequence injected at 30 ng/ul of DNA concentration (twnEx458). FMI-1::GFP was enriched in the nerve ring and the ventral nerve cord, highlighting the role of FMI-1 in axon and synapse development. We would like to note that these findings do not exclude low level of
fmi-1 expression in non-neural tissues that is below the detection threshold of our transgene.