Animals expressing NHR-49::GFP showed widespread fluorescence throughout embryonic and larval development. In adults, it was visible in all somatic tissues (such as neurons, muscle, and hypodermis), localized to both nuclei and cytoplasm, with highest expression in intestinal cells.
nhr-67DGFP exhibited a restricted expression pattern. nhr-67DGFP expression was only observed in six or seven head neurons. There was no vulval GFP expression in the nhr-67DGFP reporter line.