During postembryonic development, GFP was observed in several cell types. At the L1 stage, GFP was transiently expressed in the early M lineage. During the L2 and L3 stages, GFP was transiently detected in a group of proliferating cells surrounding the gonad, which are presumably somatic gonad cells from the Z lineage. GFP expression was also detected in three pairs of head neurons throughout postembryonic development. Two pairs of the neurons, AIM and ASK, express mls-2
(based on their location, their axon structures, co-localization with a ttx-3
::rfp (an AIY specific marker) and DiO staining which stains amphid and phasmid neurons in the head and tail).