Expr4932

strong anal dep, sphinct, vul,; moderate and sporadic bwm; no embryonic seen; re-checked embryonic 6-15 and saw none.

Expr4946

faint posterior pharynx, possibly gland or neuronal cell; looks like 4 nuclei surrounding grinder; only in one of five strains; re-checked and no GFP seen

Expr1229

Expressed from the zygotic stage in both pronuclei and in all the blastomeres throughout embryonic development. In mitotic stages, the CeSMN protein disappears from the nucleoplasm, and is re-incorporated into the newly formed nuclei. CeSMNB is present in virtually all the cell nuclei of the young nematodes.

Expr2720

Strong reporter gene expression was first observed in the hypodermal seam cells during embryogenesis and subsequently in the seam cells of all larval stages. The main hypodermal syncytium, hyp7, of larvae and young adult nematodes also displayed reporter gene activity. Strong GFP expression was observed in the toroid cells that form the developing vulva in the L4 larval stage and expression persisted into early adulthood, but was not present in the vulva of mature hermaphrodites. In addition to the hypodermal expression described above for hermaphrodites, intense reporter gene activity was also observed in the developing ray papillae of the L4 male tail. Young adult males exhibited weak expression, restricted to the hypodermis just anterior to the tail. No transgene expression was detected in the dauer larvae, but expression re-appeared approximately 12 h after dauers had been transferred to a fresh lawn of E. coli.

Expr699

Staining is first detected after fertilization in both the oocyte and sperm pronuclei shortly before the first mitotic division. After the first cleavage, the nuclei of both AB and P1 blastomeres appear to contain equivalent levels of the protein. During the first embryonic cell cycle, the protein appears to accumulate to much higher levels in the nucleus of P1 than in the AB nucleus. As AB and P1 enter mitosis, the protein is distributed throughout the cytoplasm. After the nuclei of the AB daughters re-form, both appear to have equivalent levels of the protein and similarly with the P1 daughters but at a much higher level.. During the second cell cycle the level of the protein increases in the nuclei of both P1 daughters. Late in the cell cycle the level of protein begins to decrease in both P1 daughters and in both AB daughters and in many embryos the two AB daughters have no detectable protein. After the third cleavage (8-cell stage), there is no detectable protein in any of the AB granddaughters but staining is detected throughout the P1 granddaughters. By the 12-cell stage, there is no staining in any blastomere. Peak levels of SKN-1 detected at 4-cell stage in EMS and P2. Little or no expression are detected in ABa and ABp.

Expr9638

nhr-67::gfp was expressed at all postembryonic stages in neurons of the head. nhr-67::gfp expression was not observed in uterine or vulval precursor in embryos or L1 larvae. In the L2 stage, nhr-67::gfp expression was observed in the 4 pre-VU precursor cells (Z1.ppa, Z1.ppp, Z4.aaa, Z4.aap) that become the AC and the three VU cells that give rise to the ventral uterus. Expression of nhr-67::gfp in the 4 pre-VU precursor cells appeared at or shortly after their birth. In the mid- to late-L2 stage, expression of nhr-67::gfp in the three VU cells decreased, while remaining at high levels (or possibly increasing) in the AC. During the L3 and early L4 stages, consistent and continual bright nhr-67::gfp expression was observed in the AC and more variable weak levels of expression were found in the six adjacent pi cells. nhr-67::gfp expression was very rarely detected in all 12 pi cells after their dorsal-ventral division. From early L4 through adulthood, weak expression of nhr-67::gfp was observed in all 8 UTSE cells or syncytium, suggesting that expression is turned off in all pi cells after their division and then re-expressed later in UTSE. Expression of nhr-67::gfp was observed in the UTSE before AC fusion. Bright expression of nhr-67::gfp in the AC nucleus was not observed after mid-L4, indicating that it is reduced after fusion of the AC with the UTSE syncytium.