mtm-9::gfp reporter was expressed in a broad range of tissues, including the muscle, intestine, hypodermis and neurons (including the CAN neuron in the mid-body region). Mtm-9 was also expressed in the rectal epithelial cells that are the major source of EGL-20 Wnt.
In larvae, broad MTM-1 expression is observed in L1 heads, particularly in pharynges. In adults, MTM-1 is expressed in coelomocytes and in the gonadal sheath cells. MTM-1 is broadly expressed at the apical cortex of gut cells and around gut granules. As previously reported, MTM-1 was also expressed in a few head and tail neurons.
The expression of MTM-1::GFP was seen from embryogenesis throughout larval and adult stages in many known engulfing cell types including hypodermal cells, body wall muscle cells, pharyngeal muscle cells and sheath cells. MTM-1::GFP was also observed in vulva cells, distal tip cells and coelomocytes.
mtm-3::gfp was expressed predominantly in neurons in the head with a low level of expression also detected in posterior intestinal cells which is a common artifact for GFP expression vectors.
mtm-6 is expressed in many neurons throughout the worm, the pre-anal ganglion, hypodermal cells, the anal depressor muscle, and additional non-neuronal of cells in the tail. The expression pattern is fairly constant through development.
Worms carrying the Ex-Y47H9 transgene had wild-type level broods (109 9 at 25C) in a spe-9(eb19) mutant background displayed no SPE-9 staining. Probably because of germline silencing.
EPG-9::GFP was ubiquitously expressed during embryogenesis. At postembryonic stages, epg-9 was widely expressed, including in pharyngeal muscles, neurons and intestinal cells.