Transgenic animals containing the irg-1::GFP fusion exhibited very little GFP expression when feeding on E. coli. However, expression of the irg-1::GFP transgene was robustly induced by infection with pathogenic P. aeruginosa.
In wild-type larvae, LET-23 RTK is first expressed in the vulval precursor cells in the early L2 stage. LET-23 staining is observed throughout the basolateral membrane of all six vulval precursor cells. In five of the vulval precursor cells (P3.p, P4.p, P5.p, P7.p, and P8.p), little or no LET-23 is present in the apical membrane domain. In the remaining vulval precursor cell (P6.p), LET-23 is present in both the basolateral and apical membrane domains. As P6.p divides in early L3, LET-23 staining continues to be present in both the basolateral and apical membrane domains.