- Expr2398
The
lin-23 cDNA hybridizes to a single transcript of approx. 2.8 kb with the highest level of expression in embryos and gravid adults. In situ hybridization revealed high levels of
lin-23 mRNA in the germ line of adults and early embryos. Zygotes have high levels of
lin-23 mRNA indicating the presence of maternally provided mRNA. The level of
lin-23 mRNA decreases during embryogenesis and was undetectable above background levels in larvae.
- Expr12240
LIN-23 reporter fusions are expressed in many but not all neurons (e.g., no expression is observed in commissural motor neurons). The protein shows uniform distribution along axons. LIN-23 expression is maintained throughout adulthood.
- Expr12239
Broad and possibly ubiquitous expression is first visible during gastrulation. Post-embryonically, LIN-23 reporter fusions are expressed in many but not all neurons (e.g., no expression is observed in commissural motor neurons), in body wall and enteric muscles, and in hypodermal cells. The fusion protein is excluded from the nucleus and is uniformly distributed throughout the cytoplasm. The protein also shows uniform distribution along axons. LIN-23 expression is maintained in all tissues throughout adulthood.
- Expr12833
The LIN-23 protein is present both in all blast cells of embryos and in the wild-type germline. LIN-23 is present in the germline from the tip of the distal arm to the maturing oocytes. Its abundance seems relatively similar between the distal and proximal zones; however, its localization is concentrated within cytosol and is relatively excluded from nuclei. The distal region of the gonad is a syncytial tube with the germline nuclei packed around the outside with a hollow core. We find that LIN-23 is concentrated throughout the core and in the cytosolic spaces between the nuclei and is either absent from, or present at a much reduced level, in the nuclei themselves. Within developing oocytes in the proximal region of the gonad, it is also more abundant in the cytoplasm than nuclei. An interesting observation was the differential localization of LIN-23 in the cells of the early embryo. It is generally distributed throughout the cytosol, but its localization is dynamic being differentially excluded from the nucleus for much of the cell cycle, but a fraction of it accumulates to the nuclear compartment late in the cycle shortly before cell division. In the embryos shown, the nuclear compartment accumulation of LIN-23 is seen in a late AB blastomere but is absent in a slightly younger AB blastomere. Similarly, it can be seen in both the ABa and ABp blastomeres but absent from the nuclei of the EMS and P2 blastomeres. Because the ABa and ABp blastomeres divide slightly earlier than EMS and P2, they are inevitably later in the cell cycle. However, this pattern is not lineage dependent.
- Expr15962
Endogenously tagged LIN-2, LIN-7, LIN-10 and LET-23 EGFRallow for the analysis of their localization and expression patterns in other tissues. We found that all four proteins are expressed inneurons and sensory tissue in the head, and along the ventral anddorsal nerve chords. The intestine is prone to a highdegree of autofluorescence and was excluded from the initialanalysis. Whereas LET-23 EGFR and LIN-7 overlapped minimallyin the head, LIN-2 and LIN-7 colocalized strongly in theneural ring, and the ventral and dorsal nerve chords. Ofnote, we observed that LIN-2 was more strongly expressed in theisthmus of the pharynx than LIN-7. In contrast, LIN-7 wasmore strongly expressed in the gonad and uterus than LIN-2. LIN-10 overlapped minimally with LIN-2 in the neural ring and nerve chords, and shared very little overlap with LET-23 EGFR in other neural tissues in the head of the worm. LET-23::mK2 was strongly expressed in the excretory duct cell in which it signals through the LET-60 Ras/MPK-1 ERK pathway to regulate excretory duct cell development (Abdus-Saboor et al., 2011; Yochem et al., 1997).
- Expr15965
Endogenously tagged LIN-2, LIN-7, LIN-10 and LET-23 EGFRallow for the analysis of their localization and expression patterns in other tissues. We found that all four proteins are expressed inneurons and sensory tissue in the head, and along the ventral anddorsal nerve chords. The intestine is prone to a highdegree of autofluorescence and was excluded from the initialanalysis. Whereas LET-23 EGFR and LIN-7 overlapped minimallyin the head, LIN-2 and LIN-7 colocalized strongly in theneural ring, and the ventral and dorsal nerve chords. Ofnote, we observed that LIN-2 was more strongly expressed in theisthmus of the pharynx than LIN-7. In contrast, LIN-7 wasmore strongly expressed in the gonad and uterus than LIN-2. LIN-10 overlapped minimally with LIN-2 in the neural ring and nerve chords, and shared very little overlap with LET-23 EGFR in other neural tissues in the head of the worm. LET-23::mK2 was strongly expressed in the excretory duct cell in which it signals through the LET-60 Ras/MPK-1 ERK pathway to regulate excretory duct cell development (Abdus-Saboor et al., 2011; Yochem et al., 1997).
- Expr15964
Endogenously tagged LIN-2, LIN-7, LIN-10 and LET-23 EGFRallow for the analysis of their localization and expression patterns in other tissues. We found that all four proteins are expressed inneurons and sensory tissue in the head, and along the ventral anddorsal nerve chords. The intestine is prone to a highdegree of autofluorescence and was excluded from the initialanalysis. Whereas LET-23 EGFR and LIN-7 overlapped minimallyin the head, LIN-2 and LIN-7 colocalized strongly in theneural ring, and the ventral and dorsal nerve chords. Ofnote, we observed that LIN-2 was more strongly expressed in theisthmus of the pharynx than LIN-7. In contrast, LIN-7 wasmore strongly expressed in the gonad and uterus than LIN-2. LIN-10 overlapped minimally with LIN-2 in the neural ring and nerve chords, and shared very little overlap with LET-23 EGFR in other neural tissues in the head of the worm. LET-23::mK2 was strongly expressed in the excretory duct cell in which it signals through the LET-60 Ras/MPK-1 ERK pathway to regulate excretory duct cell development (Abdus-Saboor et al., 2011; Yochem et al., 1997).
- Expr15963
Endogenously tagged LIN-2, LIN-7, LIN-10 and LET-23 EGFRallow for the analysis of their localization and expression patterns in other tissues. We found that all four proteins are expressed inneurons and sensory tissue in the head, and along the ventral anddorsal nerve chords. The intestine is prone to a highdegree of autofluorescence and was excluded from the initialanalysis. Whereas LET-23 EGFR and LIN-7 overlapped minimallyin the head, LIN-2 and LIN-7 colocalized strongly in theneural ring, and the ventral and dorsal nerve chords. Ofnote, we observed that LIN-2 was more strongly expressed in theisthmus of the pharynx than LIN-7. In contrast, LIN-7 wasmore strongly expressed in the gonad and uterus than LIN-2. LIN-10 overlapped minimally with LIN-2 in the neural ring and nerve chords, and shared very little overlap with LET-23 EGFR in other neural tissues in the head of the worm. LET-23::mK2 was strongly expressed in the excretory duct cell in which it signals through the LET-60 Ras/MPK-1 ERK pathway to regulate excretory duct cell development (Abdus-Saboor et al., 2011; Yochem et al., 1997).
- Expr15957
mNG::LIN-7, LIN-2::mK2 and mNG::LIN-10 were found to bebroadly expressed in the worm. In L3 larvae, they were prominentlyexpressed in VPCs and neurons, whereas only LIN-7 and LIN-10 weredetectable in the somatic gonad primordium. In the VPCs,endogenous mNG::LIN-7 was strongly cytosolic, frequently found atpunctae, and occasionally localized to basolateral membranes. We found that LET-23::mK2 and mNG::LIN-7 overlapped at the basolateral plasma membrane in L3 larvae (from P6.p to P6.pxx). This overlap was more apparent in the differentiated vulval cells (L4).
- Expr9316
In wild type, a
let-23:LET-23-GFP translational reporter is expressed in several non-neuronal cells and a small number of neurons, including ALA.