In late L2/early L3 larvae before the VPCs have started dividing, a translational LET-23::GFP reporter was up-regulated in the 1 x30
A; VPC P6.p, while expression faded in the other, more distal VPCs. Most of the LET-23:: GFP protein in P6.p was detected, approximately at equal levels, on the basolateral and apical plasma membranes. Only a faint and diffuse intracellular LET-23::GFP signal could be observed in the VPCs of wild-type animals. After the first round of VPC divisions, LET-23::GFP continued to be strongly expressed on the plasma membrane of the 1 x30
A; P6.p descendants.