In larvae and adults,
lim-4::GFP expression was confined to neurons in the worm's anterior ganglia; embryonic expression was not examined in detail. Expression from both transgenes was observed in the AWB neurons but not in other sensory neurons.
lim-4::GFP1 was also expressed in one RME motor neuron (RMEV), two RMD motor neurons (RMDL and RMDR), and the RID, RIV, SAA and SIA interneurons.
lim-4::GFP2 was expressed in the same neurons, except for RID and RMEV. Expression in the GABAergic RME motor neurons, which control head movement. In a subsequent study,
lim-4 was found to be expressed in SMB neurons, other than SIA neurons (Kim et al., 2015; Expr12499).