The C+C enhancer (pOK17.21) activates frequent expression in all pharyngeal muscles. the C+C enhancer also activates expression in non-muscle cells in the pharynx. Expression induced by the C+C enhancer was observed in all pharyngeal cell types including gland cells, neurons and marginal cells as well as muscles. These results define C as an organ-specific subelement.
C. briggsae, C. afra, C. angaria and Oscheius tipulae lin-3 is expressed in the anchor cell. Similar to C. elegans, lin-3 expression was also detected at a lower level in the gonad outside the anchor cell and in the pharynx.