The gene is specifically expressed in neurons, and the transcript is exclusively localized also in neurons. unc-64 transcript is not detectable in hermaphrodite specific neurons. unc-64 gene is expressed in almost all neurons constituting the head ganglion.
We noted ciliary localization of the SRBC-64 G protein-coupled receptor to the ASK cilia but also detected significant accumulation at the periciliary membrane compartment (PCMC) in wild-type animals.
Embryonic expression of the cki-2 reporter starts at about the 64-cell stage in anteriorly located blastomeres. This expression pattern apparently continues throughout embryogenesis with a gradual increase in fluorescence intensity.
A 64-bp fragment, located between 366 and 303 bp upstream of the egl-17 ATG, was sufficient to activate reporter expression in vulD and vulC cells from mid-L4 through adulthood, termed the vulDC enhancer.
Arf-1::GFP or Arf1::gfp expression was first detectable in very young embryos with as few as 48-64 cells. Expressed in the ventral nerve cord, vulva, intestine, and body wall and pharyngeal muscle tissues. Arf-1::GFP was clearly seen in the uterus, spermatheca, and gonadal sheath,
Western blot analysis of developmental stages showed that the 64 kDa NHR-60 protein was detected in embryos, during all larval stages, and in adult animals. Although ubiquitous throughout development, NHR-60 was specifically up-regulated in larval seam cells as well as in the cells of the germ line.