In hermaphrodites, P4daf-19m::GFP is expressed in IL2 neurons. In the male, P4daf- 19m::GFP is expressed in IL2 and CEM head neurons, and HOB and RnB tail neurons. The 22-bp sequence acts remotely as a
daf-19m CEM/IL2 element. In core IL2s, P4daf-19m::GFP is expressed throughout development in males and hermaphrodites. In male-specific CEMs, P4daf-19m::GFP expression commences in the mid to late L4 male, a stage that coincides with
pkd-2 expression and the onset of sexual maturity.