- Expr15265
The expression of IFB-2, IFC-1, IFD-1, IFD-2 and IFP-1 is mostly, if not completely restricted to the intestine.
- Expr15263
The expression of IFB-2, IFC-1, IFD-1, IFD-2 and IFP-1 is mostly, if not completely restricted to the intestine.
- Expr15264
The expression of IFB-2, IFC-1, IFD-1, IFD-2 and IFP-1 is mostly, if not completely restricted to the intestine.
- Expr15262
The expression of IFB-2, IFC-1, IFD-1, IFD-2 and IFP-1 is mostly, if not completely restricted to the intestine.
- Expr10040
The IFO-1 reporters labeled the perilumenal, subapical cytoplasm of intestinal cells where they co-distributed with IFB-2. To examine the expression of endogenous IFO-1, antibodies were generated against peptides 1 (position 19-39) and 2 (position 386-405). These antibodies stained the intestine in the same fashion as the reporters and anti-IFB-2 antibodies.The establishment of polarized IFO-1 distribution was examined. At a time at which the diffuse cytoplasmic IFB-2 distribution becomes apically localized, IFO-1 fluorescence was also detectable in the same region, actually slightly preceding apical IFB-2 deposition. Apical IFO-1 increases in the subsequent 1.5-fold stage were coincident with significant IFO-1 staining of the basolateral cortex. By contrast, IFB-2 was not enriched at the basolateral cortex. At the 2- to 3-fold stage, both proteins were almost completely colocalized at the apical membrane domain.
- Expr2857
Prominent expression of IFB-1 isoforms was also observed in the pharynx. Both IFB-1A and IFB-1B are expressed in marginal cells of the pharynx. IFB-1BDGFP transgenes were expressed throughout the length of the pharynx, whereas IFB-1ADGFP transgenes were expressed in a subset of pharyngeal marginal cells. In pharyngeal marginal and muscle cells, Myotactin is localized basally, whereas IFB-1ADGFP and IFB-1BDGFP were distributed throughout the apicalbasal axis. These data confirm previous reports of anti-IFB-1 staining in pharyngeal marginal cells.
- Expr2858
Anti-IFB-1 was previously reported to stain several non-epidermal cell types. Anti-IFB-1 staining in these cells was confirmed; however, in addition, consistent anti-IFB-1 staining was observed in epidermal attachment structures. The expression patterns of the GFP-tagged IFB-1 transgenes support the conclusion that this anti-IFB-1 staining in epidermal cells is specific.
- Expr15156
We found that the two isoforms IFB-1A and IFB-1B, fused to GFP and directed by their own promoters, were similarly expressed in canals, albeit differentially in other tissues. Both IFB-1A and IFB-1B were absent at lumen initiation and first appeared in laterally extending canals, where they persisted through anterior-posterior canal extension and thereafter. Double-labeling canals with cytoplasmic mCherry localized both GFP-labeled isoforms to the lumen.
- Expr12515
IFB-1::GFP is expressed in fibrous organelles (hemidesmosomes), uterine seam, and the excretory canal.
- Expr14279
Animals grown on control bacteria (OP50, control JM103) reach adulthood and display smooth periluminal localization of IFC-2a::YFP in the intestine indistinguishable from IFB-2a::CFP.