GFP expressed in the pharyngeal muscle, in pharyngeal neuron I3, and in neurons of the extrapharyngeal nervous system. Within the pharyngeal muscle, the intensity of staining is highest in the terminal bulb, lower in the isthmus, and variable in the metacorpus.
One nucleus in the anterior part of the pharyngeal metacorpus shows expression. The expression is relatively weak and rare compared to most of the patterns described here, but is reproducible and so is included. The position and singularity of expression suggests the pharyngeal interneuron I3 as the cell of interest.
Expression was detected in some neurons in the head and pharynx of larvae and adult animals. The neurons expressing Ce-TBX-2 was identified in adult animals: they were amphid sensory neurons AWB, AWC, ASJ, and pharyngeal neurons I1, I3, I5, M1, M2, M5, NSM.
Adult: strong expression in vulval epithelium, gut, head muscle, pharynx: pm1,6,7,8, I3(?), a few more pharyngeal cells (neurons? epithelium); some arcade cells, excretory duct cell, 4 neurons in the retrovesicular ganglion, rectal glands, one additional rectal cell; occasionally hyp (expresses strongly in young larvae)
ser-7::gfp DNA injected into C. elegans at 0.5-0.05 ng/Ul produced lines that exhibited marked GFP fluorescence in a number of pharyngeal neurons, including the MCs, M4, I2s, I3, M5 and occasionally in the M3s, I4, I6 and M2s. Strong GFP fluorescence was also observed in the vulval muscles.