nhr-67::gfp was expressed at all postembryonic stages in neurons of the head.
nhr-67::gfp expression was not observed in uterine or vulval precursor in embryos or L1 larvae. In the L2 stage,
nhr-67::gfp expression was observed in the 4 pre-VU precursor cells (Z1.ppa, Z1.ppp, Z4.aaa, Z4.aap) that become the AC and the three VU cells that give rise to the ventral uterus. Expression of
nhr-67::gfp in the 4 pre-VU precursor cells appeared at or shortly after their birth. In the mid- to late-L2 stage, expression of
nhr-67::gfp in the three VU cells decreased, while remaining at high levels (or possibly increasing) in the AC. During the L3 and early L4 stages, consistent and continual bright
nhr-67::gfp expression was observed in the AC and more variable weak levels of expression were found in the six adjacent pi cells.
nhr-67::gfp expression was very rarely detected in all 12 pi cells after their dorsal-ventral division. From early L4 through adulthood, weak expression of
nhr-67::gfp was observed in all 8 UTSE cells or syncytium, suggesting that expression is turned off in all pi cells after their division and then re-expressed later in UTSE. Expression of
nhr-67::gfp was observed in the UTSE before AC fusion. Bright expression of
nhr-67::gfp in the AC nucleus was not observed after mid-L4, indicating that it is reduced after fusion of the AC with the UTSE syncytium.