him-14 showed strong expression in the syncytial region of the gonad and in embryos but little or no expression in oocytes. him-14 3'UTR reporters showed no increase in oocyte expression in maturing oocytes.
The HIM-17::GFP fusion protein is strongly expressed in the germline and localizes to virtually all germline nuclei in L4 larvae and adult hermaphrodites and males. After fertilization, HIM-17::GFP is detected at low levels in early embryos until about the 16-cell stage.
All three gamete types of C. elegans (oocyte, male spermatocyte, and hermaphrodite spermatocyte) show meiosis-specific expression of HIM-3. No protein could be detected in somatic nuclei at any stage of development or in the distal tip nuclei that are undergoing mitotic divisions. A strong signal first appears in the transition zone where HIM-3 is associated with the chromatin of early meiotic prophase nuclei. Localization of HIM-3 becomes more discrete in pachytene and diplotene as the chromosomes synapse and desynapse. The protein remains associated with the chromosomes of oocytes at diakinesis; however, it could no longer be detected in postmeiotic sperm. The antibody identifies the first nuclei that are entering the pachytene stage of meiosis but does not stain the mitotic nuclei.
Faint signals were detected in L3 larva and dauer larva. No signal detected in embryos, L1, L2, L4 larva, adults, glp-4(bn2) adults, mixed-stage him-8(e1489) worms, and N2 starvation-arrested L1 larvae.
No signal detected in embryos. Faint signals detected in L1, L2, L3, L4 larva, adults, and glp-4(bn2) adults. Intense signal detected in dauer larva. Faint signal detected in mixed-stage him-8(e1489) worms, and N2 starvation-arrested L1 larvae.