HAM-2 is nuclear localized and is expressed in HSN before and during the cells migration from tail to the gonad primordium of the embryo. HAM-2 expression continues in larval HSN. HAM-2 expression in hypodermis nuclei ceases at comma stage embryo 410 min after first cleavage (before HSN migration out of the tail). Staining is seen in nuclei of HSN. HAM-2 is expressed in cells in the head.
HAM-1 is first expressed at the onset of gastrulation, approximately 100 minutes after cleavage of the zygote. At this time, HAM-1 often localizes to the periphery of cells in crescent shaped patterns that are restricted to one side of dividing cells. HAM-1 expression persists until the 1.5 fold stage of embryonic development, approximately 430 minutes after cleavage of the zygote. No HAM-1 expression can be detected later during embryonic or larval hermaphrodite development.
ham-3::gfp animals show broad gfp expression starting at gastrulation and persisting through larval and adult stages in what appear to be all cells of the worm, including HSN.