In the meiotic cells of the gonad including oocytes and in the interphase cells of 200-cell embryos, DNA topoisomerase I was localized in nuclei. At the comma stage, a more narrowed concentration of DNA topoisomerase I was observed in the nucleoli of gut cells.
GFP was shown to express in the excretory cells, coelomocytes, head neurons, hypodermal cells, and in the pharynx of C. elegans. This expression pattern was further confirmed by antibody staining using polyclonal antibodies against UCH/CeUBP130. Excretory cells were identified by the 'I-i' shape GFP expression in the head region). GFP was also shown to express in coelomocytes.
Adult: staining observed in the mature oocyte nuclei of hermaphrodites, at meiotic prophase I when the chromosomes are condensed. (Possible artifact, detected in lin-14 loss-of-function mutant strains n536n540, n355n726).