GFP-tagged synaptojanin was localized to puncta at neuromuscular junctions in the ventral nerve cord of unc-26 animals (genotype oxIs92 II; unc-26(s1710) IV).
GFP-tagged endophilin was enriched in puncta in all neuropil and colocalized with synaptobrevin, demon strating that endophilin is localized to synapses. In addition, weak protein expression was observed in the spermatheca. Furthermore, analysis of a genotype that mislocalizes synaptic vesicles suggests that endophilin may be associated with vesicles.