Coexpression of CeRhoGEF and
gpa-12 was confirmed by using CeRhoGEFp::DsRed and
gpa-12p::NLS::GFP constructs. The overlap of their expression was detected in a subset of neurons in the head, which could be the interneurons at the second pharyngeal bulb and the sensory neurons in the nerve ring area. In addition, the expression of CeRhoGEFp::DsRed or
gpa-12p::NLS::GFP was observed in neuronal processes or nuclei of ventral cord motor neurons, respectively.