Wild-type adults of either sex expressed both GLD-3L and GLD-3S. By contrast, adult
glp-1(ts) hermaphrodites, which have virtually no germline, had no detectable GLD-3, consistent with the idea that
gld-3 is expressed specifically in the germline. Furthermore, the deletion mutant,
gld-3(
q730), made no detectable GLD-3, confirming specificity of the antibodies.