Two RT-PCR products of expected sizes (747 and 825 bp) were observed at all developmental stages examined, indicating that the two gar-3
isoforms are expressed throughout development. The gar-3b
mRNA was more abundantly expressed than gar-3a
mRNA at all developmental stages. However, the relative expression ratio of the two isoforms appeared to vary between developmental stages. The gar-3a
mRNA was expressed most abundantly at the first larval stage, whereas gar-3b
mRNA was expressed most abundantly at embryonic and early larval stages.