Two RT-PCR products of expected sizes (747 and 825 bp) were observed at all developmental stages examined, indicating that the two
gar-3 isoforms are expressed throughout development. The
gar-3b mRNA was more abundantly expressed than
gar-3a mRNA at all developmental stages. However, the relative expression ratio of the two isoforms appeared to vary between developmental stages. The
gar-3a mRNA was expressed most abundantly at the first larval stage, whereas
gar-3b mRNA was expressed most abundantly at embryonic and early larval stages.