To determine the cellular expression pattern of
srf-3, constructs were generated, which fused GFP to the N terminus of SRF-3 and {beta}-galactosidase to the C terminus. Animals transgenic for either of these reporters expressed the fusion genes exclusively in the pharyngeal cells
g1 and
g2, the lateral seam cells, and the spermatheca. Expression was first visible in the seam cells of late embryos, where it persisted up to the fourth larval stage, but disappeared in adult animals. Expression in the glandular cells
g1 and
g2 started in L1 and remained visible until adulthood. In sexually mature hermaphrodites, expression was also observed in the spermatheca. In males, a similar pattern of expression was seen in
g1,
g2, and seam cells. In adult males, a strong expression signal was visible in the vas deferens.