In transgenic animals carrying the fusion gene, an
egl-19::GFP fluorescent signal was first detected in body wall muscles in 11/2-fold embryos, before the onset of embryonic muscle contraction. By the time of hatching, GFP fluorescence was found in pharyngeal muscles
pm3,
pm4,
pm5 and
pm7, in body wall muscles and in the anal depressor muscle. Expression was also found in the nervous system, including the pharyngeal neuron M4 and several neurons in the head, the ventral nerve cord and the preanal ganglion.