We found that IDPA-3, IDPB-3, IDPC-1, FIPR-4, and NSPB-12 are expressed exclusively in association with the pharynx and overlap in their localization with the pharynx cuticle. Briefly, tagged IDPA-3 was enriched in the grinder, overlapping the CFW-stained component and lining of the terminal bulb cuticle. In addition, we observed enrichment of tagged IDPA-3 in the presumptive ECM that lies between the terminal bulb and the intestinal valve. Tagged IDPB-3 was expressed weakly and localized exclusively to the
pm6 cells and material surrounding the CFW-stained grinder. Tagged IDPC-1 had a similar pattern to that of tagged ABU-14; associating with both the anterior and posterior components of the pharyngeal cuticle. However, tagged ABU-14 appears to localize adjacent to CFW-stained components whereas tagged IDPC-1 overlaps CFW-stained components. Tagged NSPB-12 localized to the anterior pharynx cuticle components exclusively, including that of the buccal cavity, flaps, and anterior channels. Tagged FIPR-4 localized to both anterior and posterior pharynx cuticle components (but not the grinder teeth proper) and the presumptive pharynx-intestinal valve ECM.