glna-3P::gfp expression, driven by a 1422-bp segment in the 5' upstream region of the
glna-3 gene, was localized in head neurons. Specifically,
glna-3P::gfp expression and a dye filling assay, which stains chemosensory amphid neurons, showed that
glna-3 is expressed in AWB neurons, consistent with previous findings that
eat-4/vGlut1 is expressed in AWB neurons. By contrast, ASI, ADL, ASK, ASH, and ASJ neurons did not express
glna-3P::gfp.However, the
glna-3P::gfp reporter used in this study was expressed in a limited number of head neurons compared to that of previously reported. This is presumably because the promoter construct used here did not include the first intron sequence.