Examination of transgenic animals carrying pGP16 using confocal microscopy showed that EAT-16 is expressed in most or all neurons, including the hermaphrodite specific neuron (HSN), as well as in the vulval and pharyngeal muscles. Expression was also occasionally seen in the spermatheca and body wall muscles.
Both EAT-17 and RAB-6.2 are highly expressed in the pharyngeal muscle. RAB-6-2 is also highly expressed in neurons. EAT-17 expression was also detected in the intestine and vulva.
Both EAT-17 and RAB-6.2 are highly expressed in the pharyngeal muscle. RAB-6-2 is also highly expressed in neurons. EAT-17 expression was also detected in the intestine and vulva.
We confirmed that eat-4 (481 bp sequence located downstream of the translation start site: eat-4p12) (22) and unc-8 (293 bp sequence located ∼197 bp upstream of the translation start site: unc-8Δ7p) were indeed expressed in all six IL1 neurons.
We confirmed that eat-4 (481 bp sequence located downstream of the translation start site: eat-4p12) (22) and unc-8 (293 bp sequence located ∼197 bp upstream of the translation start site: unc-8Δ7p) were indeed expressed in all six IL1 neurons.
Expression of mpk-1::gfp was observed in the rectal epithelial cells, including F and U, in 100% of wild type L1 (16/16), L2 (19/19), L3 (15/15), L4 (21/21) and adult (16/16)worms.