tbx-2 transcripts was detected starting at the 4E stage (~102 cell stage, 143 min after the 4-cell stage), and with mid-gastrula expression observed by in situ hybridization (Shin-i and Kohara, http://nematode.lab.nig.ac.jp/).
At the mid-L2 stage, the VD neurons form synapses (Hallam and Jin, 1998; Kurup and Jin, 2015; Sulston, 1976), and KIN-20 expression is identical in DD and VD cell bodies. At the L2 stage, KIN-20 is also highly expressed in the nuclei of epidermal cells and is visible in the nuclei of motor neurons. Expression tends to peak at the end of each larval stage, consistent with expression of other heterochronic genes (Rhodehouse et al., 2018).