dpy-14 expression starts at the "16 E cell period", a stage when the embryo consists of 224N-500 cells and 4.25N-5.5 h have elapsed since fertilization. As embryogenesis progresses, expression of
dpy-14 becomes clearly hypodermal and is mainly localized to
hyp7. In addition to embryonic expression, some GFP fluorescence was observed in the
hyp7 syncytium of L1 worms, but not at later larval stages or in adults. This L1 expression is probably an artefact due to the relatively long half-life of GFP. GFP expression of
dpy-14 at the lima bean stage was mainly localized to cells of AB lineage that may be grouped into two categories based on their potential to differentiate into hypodermal cells only (AB.alaa, AB.alap, AB.arpa, AB.arap, AB.arpp and AB.alpa), or into either hypodermal cells or ciliated neurons (AB.praa, AB.prap, AB.plpp, AB.plpa and AB.alpp). At the plum stage, expression was localized to
hyp7 nuclei and to some amphids (ADFL, AFDL, ASEL, ASHL, ASJL, ASKL and RIAL) and amphid support cells (IlshL and OLQsoVL).