The expression of
Ce-wts-1 begins at the comma stage and proceeds during the entire larval and adult stages. Ce- WTS-1::GFP was detectable in many tissues, including pharynx, gut, vulval, spermathecal, and seam cells. The subcellular localization of Ce-WTS-1 appears to be close to the membrane or membrane-associated, i.e., in gut apical membrane, vulval cell membrane, spermathecal cell membrane and seam cell membrane. However, the 'DAS' transmembrane domain prediction program predicted no transmembrane domain in CE-WTS-1. In addition, neither fly Warts or human LATS1 contains transmembrane domains. Therefore, Ce-WTS-1 is likely accumulated intracellularly near the cell membrane and may interact with membrane or membrane associated proteins.