din-1S::gfp localized to the nuclei of most cell types. Detected in hypodermis, seam, intestine, and somatic gonad including the distal tip cells,
din-1S was also expressed in neurons, vulval precursors, body wall muscle, and pharynx, all tissues with heterochronic phenotypes or remodeled during dauer. Expression was first detected in a few nuclei by the comma stage of embryogenesis. By hatch,
din-1S was widely expressed, albeit weakly. Overall expression in most tissues was detected at various levels into adult and in dauer larvae. Interestingly, in late L1 and L2 larvae
din-1S was often (75%, n = 16) highly expressed in the XXX cells, specialized neuroendocrine cells proposed to be a site of synthesis for the
daf-9-produced hormone.