As previously observed for the A- and C-isoform translational fusion constructs, young adults containing a wild-type copy of the
daf-19 gene demonstrate robust expression of the
mapk-15 transcriptional-fusion construct in head and tail CSNs of both hermaphrodite and male animals. However, expression of the
mapk-15 transcriptional construct was additionally found in the head neurons AFD, ADE, and ASG, as distinguished from other neurons based on their proximity to known dye-filled neurons.